Journal: iScience

Article Title: Complement is primarily activated in the lung in a mouse model of severe COVID-19

doi: 10.1016/j.isci.2025.111930

Figure Lengend Snippet:

Article Snippet: Following electrophoresis and transfer to PVDF membranes, samples were blocked with 5% milk (2.5% milk for C4) and incubated with primary antibody anti-mouse C3 (1:500, cat#55463, MP Biomedicals), goat anti-human FB (1:500, cat#A311, Quidel), anti-mouse C9 (1:500, cat#HM1134, Hycult, or anti-mouse C4 (1:200, cat#ab11863, Abcam) overnight.

Techniques: Virus, Cell Culture, Recombinant, Sample Prep, RNAscope, Enzyme-linked Immunosorbent Assay, Software

Journal: American Journal of Translational Research

Article Title: Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori

doi:

Figure Lengend Snippet: A: Immunohistochemical staining of HSP-90, HSP-70, HSP-60, HSP-27 proteins in gastric tissue cells of normal people, gastritis patients infected by H. pylori, gastritis patients and gastric cancer patients. All the pictures are in high-power fields (×400). B: Quantification of the expression of HSP-90, HSP-70, HSP-60, HSP-27 in four kind of gastric tissue cells.

Article Snippet: Immunostainings for HSP-27, HSP-60, HSP-70 and HSP-90 in gastric tissues of normal individuals, gastritis caused by H. pylori , gastritis and gastric cancer tissues were performed on paraffin-embedded tissue sections using commercially available goat anti-human HSP-27, HSP-60, HSP-70 and HSP-90 (BD, Franklin Lakes, NJ, USA) primary antibodies.

Techniques: Immunohistochemical staining, Staining, Infection, Expressing

Journal: American Journal of Translational Research

Article Title: Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori

doi:

Figure Lengend Snippet: HSP-90, HSP-70, HSP-60, HSP-27 proteins in RGM-1 cells (A), macrophages (C), Dendritic cells (E) treated with H. pylori infection or heat shock were analyzed by western blotting. (A, C, E) Results of western blotting, α-tubulin as a standard calibration; (B, D, F) Quantified assay of HSP-90, HSP-70, HSP-60, HSP-27 analyzed by SPSS 16.0, with asterisks indicating the pairs of values compared for which significant differences were observed was presented as mean ± SEM. (n = 3 compared with the control group, *P≤0.05 vs normal or control).

Article Snippet: Immunostainings for HSP-27, HSP-60, HSP-70 and HSP-90 in gastric tissues of normal individuals, gastritis caused by H. pylori , gastritis and gastric cancer tissues were performed on paraffin-embedded tissue sections using commercially available goat anti-human HSP-27, HSP-60, HSP-70 and HSP-90 (BD, Franklin Lakes, NJ, USA) primary antibodies.

Techniques: Infection, Western Blot

Journal: American Journal of Translational Research

Article Title: Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori

doi:

Figure Lengend Snippet: HSP-90, HSP-70, HSP-60, HSP-27 proteins in exosomes obtained from cell supernatant of RGM-1 cells (A), macrophages (C), Dendritic cells (E) stimulated with H. pylori or heat shock were analyzed by western blotting. (A, C, E) Results of western blotting, α-tubulin as a standard calibration; (B, D, F) Quantified assay of HSP-90, HSP-70, HSP-60, HSP-27 analyzed by SPSS 16.0, with asterisks indicating the pairs of values compared for which significant differences were observed was presented as mean ± SEM. (n = 3 compared with the control group, *P≤0.05 vs normal or control).

Article Snippet: Immunostainings for HSP-27, HSP-60, HSP-70 and HSP-90 in gastric tissues of normal individuals, gastritis caused by H. pylori , gastritis and gastric cancer tissues were performed on paraffin-embedded tissue sections using commercially available goat anti-human HSP-27, HSP-60, HSP-70 and HSP-90 (BD, Franklin Lakes, NJ, USA) primary antibodies.

Techniques: Western Blot

Journal: American Journal of Translational Research

Article Title: Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori

doi:

Figure Lengend Snippet: HSP-90α, HSP-70, HSP-60, HSP-27 in cell culture supernatants of RGM-1 cells, macrophages, Dendritic cells infected with H. pylori were determined by ELISA method. Each symbol per condition represents the data obtained from one tests. Horizontal lines show the median values of 15 experiments. **P≤0.05 compared with medium alone (Friedman test and Dunn’s multiple comparison test).

Article Snippet: Immunostainings for HSP-27, HSP-60, HSP-70 and HSP-90 in gastric tissues of normal individuals, gastritis caused by H. pylori , gastritis and gastric cancer tissues were performed on paraffin-embedded tissue sections using commercially available goat anti-human HSP-27, HSP-60, HSP-70 and HSP-90 (BD, Franklin Lakes, NJ, USA) primary antibodies.

Techniques: Cell Culture, Infection, Enzyme-linked Immunosorbent Assay

Journal: American Journal of Translational Research

Article Title: Comparative analysis of the interaction of HSPs in dendritic cells, macrophages, RGM-1 cells infected by Helicobacter pylori

doi:

Figure Lengend Snippet: Analysis of HSP-90α, HSP-70, HSP-60 and HSP-27 in serum of normal individual, gastritis patients, H. pylori gastritis patients and gastric cancer patients was detected by ELISA method. Each symbol per condition represents the data obtained from one patients. Horizontal lines show the median values of 15 experiments. **P≤0.05 compared with medium alone (Friedman test and Dunn’s multiple comparison test).

Article Snippet: Immunostainings for HSP-27, HSP-60, HSP-70 and HSP-90 in gastric tissues of normal individuals, gastritis caused by H. pylori , gastritis and gastric cancer tissues were performed on paraffin-embedded tissue sections using commercially available goat anti-human HSP-27, HSP-60, HSP-70 and HSP-90 (BD, Franklin Lakes, NJ, USA) primary antibodies.

Techniques: Enzyme-linked Immunosorbent Assay